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biotin 1d4c5 fluidigm 3150008b il 10 jes3 biolegend 501502 biotin  (fluidigm)


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    fluidigm biotin 1d4c5 fluidigm 3150008b il 10 jes3 biolegend 501502 biotin
    Biotin 1d4c5 Fluidigm 3150008b Il 10 Jes3 Biolegend 501502 Biotin, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxpar+x8+antibody+labeling+kit+fluidigm/Maxpar+X8+Antibody+Labeling+Kit%2C+149Sm/us12521393-2842-140-142
    Average 93 stars, based on 7 article reviews
    biotin 1d4c5 fluidigm 3150008b il 10 jes3 biolegend 501502 biotin - by Bioz Stars, 2026-09
    93/100 stars

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    Cytometry:

    Article Title: An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat F(ab’)2 Anti-Human Kappa-UNLB Southern Biotech Cat#2062-01; RRID: AB_2795736 All antibodies used for cytometry Various Table S1 Biological Samples Leukocyte reduction chamber from human blood Stanford Blood Center stanfordbloodcenter.org Whole blood from healthy donors Obtained under informed consent with IRB approval n/a Human tonsil Stanford Hospital, adult tonsilectomy from adults with obstructive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com Chemicals, Peptides, and Recombinant Proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 RPMI 1640 media Thermo Fisher Scientific Cat#21-870-092 GlutaMAX supplement Thermo Fisher Scientific Cat#35050-061 Fetal bovine serum USDA approved lot Omega Scientific, Inc. Cat#FB-01 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Recombinant human CD40L BioLegend Cat#591704 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Cell-ID Intercalator-Ir Fluidigm Cat#201192A Cell-ID Cisplatin Fluidigm Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Fluidigm Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Streptavidin Particles Plus BD Biosciences Cat#557812 Critical Commercial Assays Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 Maxpar X8 Antibody Labeling Kit Fluidigm Cat#201176B IMag Cell Separation Magnet BD Biosciences Cat#552311 QIAamp DNA Micro Kit QIAGEN Cat#56304 immunoSeq Human B Cell - survey resolution Adaptive adaptivebiotech.com Deposited Data Mass cytometry data This study flowrepository.org/id/FR-FCM-Z2MA flowrepository.org/id/FR-FCM-Z2MC Software and Algorithms RStudio Rstudio rstudio.com MATLAB MathWorks mathworks.com/products/MATLAB.html Cytobank Cytobank cytobank.org CellEngine Primity Bio cellengine.com Other Custom code for analysis This study github.com/davidrglass/atlas Immunity 53, 217–232.e1–e5, July 14, 2020 e1 ll OPEN ACCESS Resource ..

    Recombinant:

    Article Title: An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat F(ab’)2 Anti-Human Kappa-UNLB Southern Biotech Cat#2062-01; RRID: AB_2795736 All antibodies used for cytometry Various Table S1 Biological Samples Leukocyte reduction chamber from human blood Stanford Blood Center stanfordbloodcenter.org Whole blood from healthy donors Obtained under informed consent with IRB approval n/a Human tonsil Stanford Hospital, adult tonsilectomy from adults with obstructive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com Chemicals, Peptides, and Recombinant Proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 RPMI 1640 media Thermo Fisher Scientific Cat#21-870-092 GlutaMAX supplement Thermo Fisher Scientific Cat#35050-061 Fetal bovine serum USDA approved lot Omega Scientific, Inc. Cat#FB-01 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Recombinant human CD40L BioLegend Cat#591704 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Cell-ID Intercalator-Ir Fluidigm Cat#201192A Cell-ID Cisplatin Fluidigm Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Fluidigm Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Streptavidin Particles Plus BD Biosciences Cat#557812 Critical Commercial Assays Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 Maxpar X8 Antibody Labeling Kit Fluidigm Cat#201176B IMag Cell Separation Magnet BD Biosciences Cat#552311 QIAamp DNA Micro Kit QIAGEN Cat#56304 immunoSeq Human B Cell - survey resolution Adaptive adaptivebiotech.com Deposited Data Mass cytometry data This study flowrepository.org/id/FR-FCM-Z2MA flowrepository.org/id/FR-FCM-Z2MC Software and Algorithms RStudio Rstudio rstudio.com MATLAB MathWorks mathworks.com/products/MATLAB.html Cytobank Cytobank cytobank.org CellEngine Primity Bio cellengine.com Other Custom code for analysis This study github.com/davidrglass/atlas Immunity 53, 217–232.e1–e5, July 14, 2020 e1 ll OPEN ACCESS Resource ..

    Blocking Assay:

    Article Title: An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat F(ab’)2 Anti-Human Kappa-UNLB Southern Biotech Cat#2062-01; RRID: AB_2795736 All antibodies used for cytometry Various Table S1 Biological Samples Leukocyte reduction chamber from human blood Stanford Blood Center stanfordbloodcenter.org Whole blood from healthy donors Obtained under informed consent with IRB approval n/a Human tonsil Stanford Hospital, adult tonsilectomy from adults with obstructive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com Chemicals, Peptides, and Recombinant Proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 RPMI 1640 media Thermo Fisher Scientific Cat#21-870-092 GlutaMAX supplement Thermo Fisher Scientific Cat#35050-061 Fetal bovine serum USDA approved lot Omega Scientific, Inc. Cat#FB-01 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Recombinant human CD40L BioLegend Cat#591704 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Cell-ID Intercalator-Ir Fluidigm Cat#201192A Cell-ID Cisplatin Fluidigm Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Fluidigm Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Streptavidin Particles Plus BD Biosciences Cat#557812 Critical Commercial Assays Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 Maxpar X8 Antibody Labeling Kit Fluidigm Cat#201176B IMag Cell Separation Magnet BD Biosciences Cat#552311 QIAamp DNA Micro Kit QIAGEN Cat#56304 immunoSeq Human B Cell - survey resolution Adaptive adaptivebiotech.com Deposited Data Mass cytometry data This study flowrepository.org/id/FR-FCM-Z2MA flowrepository.org/id/FR-FCM-Z2MC Software and Algorithms RStudio Rstudio rstudio.com MATLAB MathWorks mathworks.com/products/MATLAB.html Cytobank Cytobank cytobank.org CellEngine Primity Bio cellengine.com Other Custom code for analysis This study github.com/davidrglass/atlas Immunity 53, 217–232.e1–e5, July 14, 2020 e1 ll OPEN ACCESS Resource ..

    Staining:

    Article Title: An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat F(ab’)2 Anti-Human Kappa-UNLB Southern Biotech Cat#2062-01; RRID: AB_2795736 All antibodies used for cytometry Various Table S1 Biological Samples Leukocyte reduction chamber from human blood Stanford Blood Center stanfordbloodcenter.org Whole blood from healthy donors Obtained under informed consent with IRB approval n/a Human tonsil Stanford Hospital, adult tonsilectomy from adults with obstructive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com Chemicals, Peptides, and Recombinant Proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 RPMI 1640 media Thermo Fisher Scientific Cat#21-870-092 GlutaMAX supplement Thermo Fisher Scientific Cat#35050-061 Fetal bovine serum USDA approved lot Omega Scientific, Inc. Cat#FB-01 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Recombinant human CD40L BioLegend Cat#591704 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Cell-ID Intercalator-Ir Fluidigm Cat#201192A Cell-ID Cisplatin Fluidigm Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Fluidigm Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Streptavidin Particles Plus BD Biosciences Cat#557812 Critical Commercial Assays Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 Maxpar X8 Antibody Labeling Kit Fluidigm Cat#201176B IMag Cell Separation Magnet BD Biosciences Cat#552311 QIAamp DNA Micro Kit QIAGEN Cat#56304 immunoSeq Human B Cell - survey resolution Adaptive adaptivebiotech.com Deposited Data Mass cytometry data This study flowrepository.org/id/FR-FCM-Z2MA flowrepository.org/id/FR-FCM-Z2MC Software and Algorithms RStudio Rstudio rstudio.com MATLAB MathWorks mathworks.com/products/MATLAB.html Cytobank Cytobank cytobank.org CellEngine Primity Bio cellengine.com Other Custom code for analysis This study github.com/davidrglass/atlas Immunity 53, 217–232.e1–e5, July 14, 2020 e1 ll OPEN ACCESS Resource ..

    Antibody Labeling:

    Article Title: An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat F(ab’)2 Anti-Human Kappa-UNLB Southern Biotech Cat#2062-01; RRID: AB_2795736 All antibodies used for cytometry Various Table S1 Biological Samples Leukocyte reduction chamber from human blood Stanford Blood Center stanfordbloodcenter.org Whole blood from healthy donors Obtained under informed consent with IRB approval n/a Human tonsil Stanford Hospital, adult tonsilectomy from adults with obstructive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com Chemicals, Peptides, and Recombinant Proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 RPMI 1640 media Thermo Fisher Scientific Cat#21-870-092 GlutaMAX supplement Thermo Fisher Scientific Cat#35050-061 Fetal bovine serum USDA approved lot Omega Scientific, Inc. Cat#FB-01 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Recombinant human CD40L BioLegend Cat#591704 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Cell-ID Intercalator-Ir Fluidigm Cat#201192A Cell-ID Cisplatin Fluidigm Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Fluidigm Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Streptavidin Particles Plus BD Biosciences Cat#557812 Critical Commercial Assays Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 Maxpar X8 Antibody Labeling Kit Fluidigm Cat#201176B IMag Cell Separation Magnet BD Biosciences Cat#552311 QIAamp DNA Micro Kit QIAGEN Cat#56304 immunoSeq Human B Cell - survey resolution Adaptive adaptivebiotech.com Deposited Data Mass cytometry data This study flowrepository.org/id/FR-FCM-Z2MA flowrepository.org/id/FR-FCM-Z2MC Software and Algorithms RStudio Rstudio rstudio.com MATLAB MathWorks mathworks.com/products/MATLAB.html Cytobank Cytobank cytobank.org CellEngine Primity Bio cellengine.com Other Custom code for analysis This study github.com/davidrglass/atlas Immunity 53, 217–232.e1–e5, July 14, 2020 e1 ll OPEN ACCESS Resource ..

    Article Title: The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER BD Rhapsody cDNA Kit BectonDickinson Cat# 633773 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32851 High Sensitivity DNA Kit Agilent Cat# 5067-4626 NovaSeq 6000 S2 Reagent Kit (200 cycles) Illumina Cat# 20040326 NovaSeq 6000 S4 Reagent Kit (200 cycles) Illumina Cat# 20028313 Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat# 1000121 Chromium Next GEM Chip G Single Cell Kit 10x Genomics Cat# 1000120 Single Index Kit T Set A 10x Genomics Cat# 1000213 TruSeq Stranded Total RNA with Ribo-Zero Globin Illumina Cat# 20020613 Maxpar X8 Antibody Labeling Kit Fluidigm Cat# 201146B Quick-DNA Microprep Kit Zymo Research Cat# D3020 EZ DNA Methylation-Gold Kit Zymo Research Cat# D5006 Infinium MethylationEPIC BeadChip Kit Illumina Cat# WG-317-1002 Zombie Aqua Fixable Viability Kit BioLegend Cat# 423101 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit Becton, Dickinson Cat# 554714 SeraSpot Anti-SARS-CoV-2 IgG microarray Seramun Diagnostica SP-015-4 G-S12 RUO Deposited data scRNA-seq raw data This paper EGAS00001007461, EGAS50000000203 bulk RNA-seq raw data This paper EGAS00001007461 processed scRNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa processed bulk RNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa DNA methylation data This paper GSE270901 Code for DNA methylation data analysis This paper https://github.com/knollr/COVID_Dexa Software and algorithms Bcl2fastq2 Illumina v2.20 STAR Dobin et al.47 v2.7.3a Cutadapt Martin et al.48 v1.16 Dropseq-tools https://github.com/broadinstitute/Drop- seq/ v2.0.0 fastQC Andrews et al.49 0.11.8 10x Genomics Cell Ranger (Software) Zheng et al.,50 10X Genomics 7.0.0 R (bulk and scRNA-seq blood analysis) https://www.cran.r-project.org v4.1.0 R (DNA methylation analysis) https://www.cran.r-project.org v4.2.1 R (scRNA-seq BAL analysis) https://www.cran.r-project.org v4.3.1 Seurat (scRNA-seq blood, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.3.0 Seurat (scRNA-seq BAL, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.4.0 Harmony (R package) Korsunsky et al.54 v0.1.0 ClusterProfiler (R package) Yu et al.55 v4.0.5 batchelor (R package) Haghverdi et al.56 v1.16.0 AUCell (R package) Aibar et al.57 v1.22.0 DESeq2 (R package) Love et al.58 v1.32.0 (Continued on next page) ll OPEN ACCESS Cell 187, 1–18.e1–e12, August 8, 2024 e3 Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article

    Mass Cytometry:

    Article Title: An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat F(ab’)2 Anti-Human Kappa-UNLB Southern Biotech Cat#2062-01; RRID: AB_2795736 All antibodies used for cytometry Various Table S1 Biological Samples Leukocyte reduction chamber from human blood Stanford Blood Center stanfordbloodcenter.org Whole blood from healthy donors Obtained under informed consent with IRB approval n/a Human tonsil Stanford Hospital, adult tonsilectomy from adults with obstructive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com Chemicals, Peptides, and Recombinant Proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 RPMI 1640 media Thermo Fisher Scientific Cat#21-870-092 GlutaMAX supplement Thermo Fisher Scientific Cat#35050-061 Fetal bovine serum USDA approved lot Omega Scientific, Inc. Cat#FB-01 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Recombinant human CD40L BioLegend Cat#591704 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Cell-ID Intercalator-Ir Fluidigm Cat#201192A Cell-ID Cisplatin Fluidigm Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Fluidigm Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Streptavidin Particles Plus BD Biosciences Cat#557812 Critical Commercial Assays Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 Maxpar X8 Antibody Labeling Kit Fluidigm Cat#201176B IMag Cell Separation Magnet BD Biosciences Cat#552311 QIAamp DNA Micro Kit QIAGEN Cat#56304 immunoSeq Human B Cell - survey resolution Adaptive adaptivebiotech.com Deposited Data Mass cytometry data This study flowrepository.org/id/FR-FCM-Z2MA flowrepository.org/id/FR-FCM-Z2MC Software and Algorithms RStudio Rstudio rstudio.com MATLAB MathWorks mathworks.com/products/MATLAB.html Cytobank Cytobank cytobank.org CellEngine Primity Bio cellengine.com Other Custom code for analysis This study github.com/davidrglass/atlas Immunity 53, 217–232.e1–e5, July 14, 2020 e1 ll OPEN ACCESS Resource ..

    Software:

    Article Title: An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat F(ab’)2 Anti-Human Kappa-UNLB Southern Biotech Cat#2062-01; RRID: AB_2795736 All antibodies used for cytometry Various Table S1 Biological Samples Leukocyte reduction chamber from human blood Stanford Blood Center stanfordbloodcenter.org Whole blood from healthy donors Obtained under informed consent with IRB approval n/a Human tonsil Stanford Hospital, adult tonsilectomy from adults with obstructive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com Chemicals, Peptides, and Recombinant Proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 RPMI 1640 media Thermo Fisher Scientific Cat#21-870-092 GlutaMAX supplement Thermo Fisher Scientific Cat#35050-061 Fetal bovine serum USDA approved lot Omega Scientific, Inc. Cat#FB-01 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Recombinant human CD40L BioLegend Cat#591704 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Cell-ID Intercalator-Ir Fluidigm Cat#201192A Cell-ID Cisplatin Fluidigm Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Fluidigm Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Streptavidin Particles Plus BD Biosciences Cat#557812 Critical Commercial Assays Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 Maxpar X8 Antibody Labeling Kit Fluidigm Cat#201176B IMag Cell Separation Magnet BD Biosciences Cat#552311 QIAamp DNA Micro Kit QIAGEN Cat#56304 immunoSeq Human B Cell - survey resolution Adaptive adaptivebiotech.com Deposited Data Mass cytometry data This study flowrepository.org/id/FR-FCM-Z2MA flowrepository.org/id/FR-FCM-Z2MC Software and Algorithms RStudio Rstudio rstudio.com MATLAB MathWorks mathworks.com/products/MATLAB.html Cytobank Cytobank cytobank.org CellEngine Primity Bio cellengine.com Other Custom code for analysis This study github.com/davidrglass/atlas Immunity 53, 217–232.e1–e5, July 14, 2020 e1 ll OPEN ACCESS Resource ..

    Article Title: The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER BD Rhapsody cDNA Kit BectonDickinson Cat# 633773 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32851 High Sensitivity DNA Kit Agilent Cat# 5067-4626 NovaSeq 6000 S2 Reagent Kit (200 cycles) Illumina Cat# 20040326 NovaSeq 6000 S4 Reagent Kit (200 cycles) Illumina Cat# 20028313 Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat# 1000121 Chromium Next GEM Chip G Single Cell Kit 10x Genomics Cat# 1000120 Single Index Kit T Set A 10x Genomics Cat# 1000213 TruSeq Stranded Total RNA with Ribo-Zero Globin Illumina Cat# 20020613 Maxpar X8 Antibody Labeling Kit Fluidigm Cat# 201146B Quick-DNA Microprep Kit Zymo Research Cat# D3020 EZ DNA Methylation-Gold Kit Zymo Research Cat# D5006 Infinium MethylationEPIC BeadChip Kit Illumina Cat# WG-317-1002 Zombie Aqua Fixable Viability Kit BioLegend Cat# 423101 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit Becton, Dickinson Cat# 554714 SeraSpot Anti-SARS-CoV-2 IgG microarray Seramun Diagnostica SP-015-4 G-S12 RUO Deposited data scRNA-seq raw data This paper EGAS00001007461, EGAS50000000203 bulk RNA-seq raw data This paper EGAS00001007461 processed scRNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa processed bulk RNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa DNA methylation data This paper GSE270901 Code for DNA methylation data analysis This paper https://github.com/knollr/COVID_Dexa Software and algorithms Bcl2fastq2 Illumina v2.20 STAR Dobin et al.47 v2.7.3a Cutadapt Martin et al.48 v1.16 Dropseq-tools https://github.com/broadinstitute/Drop- seq/ v2.0.0 fastQC Andrews et al.49 0.11.8 10x Genomics Cell Ranger (Software) Zheng et al.,50 10X Genomics 7.0.0 R (bulk and scRNA-seq blood analysis) https://www.cran.r-project.org v4.1.0 R (DNA methylation analysis) https://www.cran.r-project.org v4.2.1 R (scRNA-seq BAL analysis) https://www.cran.r-project.org v4.3.1 Seurat (scRNA-seq blood, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.3.0 Seurat (scRNA-seq BAL, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.4.0 Harmony (R package) Korsunsky et al.54 v0.1.0 ClusterProfiler (R package) Yu et al.55 v4.0.5 batchelor (R package) Haghverdi et al.56 v1.16.0 AUCell (R package) Aibar et al.57 v1.22.0 DESeq2 (R package) Love et al.58 v1.32.0 (Continued on next page) ll OPEN ACCESS Cell 187, 1–18.e1–e12, August 8, 2024 e3 Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article

    Methylation:

    Article Title: The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER BD Rhapsody cDNA Kit BectonDickinson Cat# 633773 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32851 High Sensitivity DNA Kit Agilent Cat# 5067-4626 NovaSeq 6000 S2 Reagent Kit (200 cycles) Illumina Cat# 20040326 NovaSeq 6000 S4 Reagent Kit (200 cycles) Illumina Cat# 20028313 Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat# 1000121 Chromium Next GEM Chip G Single Cell Kit 10x Genomics Cat# 1000120 Single Index Kit T Set A 10x Genomics Cat# 1000213 TruSeq Stranded Total RNA with Ribo-Zero Globin Illumina Cat# 20020613 Maxpar X8 Antibody Labeling Kit Fluidigm Cat# 201146B Quick-DNA Microprep Kit Zymo Research Cat# D3020 EZ DNA Methylation-Gold Kit Zymo Research Cat# D5006 Infinium MethylationEPIC BeadChip Kit Illumina Cat# WG-317-1002 Zombie Aqua Fixable Viability Kit BioLegend Cat# 423101 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit Becton, Dickinson Cat# 554714 SeraSpot Anti-SARS-CoV-2 IgG microarray Seramun Diagnostica SP-015-4 G-S12 RUO Deposited data scRNA-seq raw data This paper EGAS00001007461, EGAS50000000203 bulk RNA-seq raw data This paper EGAS00001007461 processed scRNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa processed bulk RNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa DNA methylation data This paper GSE270901 Code for DNA methylation data analysis This paper https://github.com/knollr/COVID_Dexa Software and algorithms Bcl2fastq2 Illumina v2.20 STAR Dobin et al.47 v2.7.3a Cutadapt Martin et al.48 v1.16 Dropseq-tools https://github.com/broadinstitute/Drop- seq/ v2.0.0 fastQC Andrews et al.49 0.11.8 10x Genomics Cell Ranger (Software) Zheng et al.,50 10X Genomics 7.0.0 R (bulk and scRNA-seq blood analysis) https://www.cran.r-project.org v4.1.0 R (DNA methylation analysis) https://www.cran.r-project.org v4.2.1 R (scRNA-seq BAL analysis) https://www.cran.r-project.org v4.3.1 Seurat (scRNA-seq blood, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.3.0 Seurat (scRNA-seq BAL, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.4.0 Harmony (R package) Korsunsky et al.54 v0.1.0 ClusterProfiler (R package) Yu et al.55 v4.0.5 batchelor (R package) Haghverdi et al.56 v1.16.0 AUCell (R package) Aibar et al.57 v1.22.0 DESeq2 (R package) Love et al.58 v1.32.0 (Continued on next page) ll OPEN ACCESS Cell 187, 1–18.e1–e12, August 8, 2024 e3 Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article

    Microarray:

    Article Title: The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER BD Rhapsody cDNA Kit BectonDickinson Cat# 633773 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32851 High Sensitivity DNA Kit Agilent Cat# 5067-4626 NovaSeq 6000 S2 Reagent Kit (200 cycles) Illumina Cat# 20040326 NovaSeq 6000 S4 Reagent Kit (200 cycles) Illumina Cat# 20028313 Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat# 1000121 Chromium Next GEM Chip G Single Cell Kit 10x Genomics Cat# 1000120 Single Index Kit T Set A 10x Genomics Cat# 1000213 TruSeq Stranded Total RNA with Ribo-Zero Globin Illumina Cat# 20020613 Maxpar X8 Antibody Labeling Kit Fluidigm Cat# 201146B Quick-DNA Microprep Kit Zymo Research Cat# D3020 EZ DNA Methylation-Gold Kit Zymo Research Cat# D5006 Infinium MethylationEPIC BeadChip Kit Illumina Cat# WG-317-1002 Zombie Aqua Fixable Viability Kit BioLegend Cat# 423101 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit Becton, Dickinson Cat# 554714 SeraSpot Anti-SARS-CoV-2 IgG microarray Seramun Diagnostica SP-015-4 G-S12 RUO Deposited data scRNA-seq raw data This paper EGAS00001007461, EGAS50000000203 bulk RNA-seq raw data This paper EGAS00001007461 processed scRNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa processed bulk RNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa DNA methylation data This paper GSE270901 Code for DNA methylation data analysis This paper https://github.com/knollr/COVID_Dexa Software and algorithms Bcl2fastq2 Illumina v2.20 STAR Dobin et al.47 v2.7.3a Cutadapt Martin et al.48 v1.16 Dropseq-tools https://github.com/broadinstitute/Drop- seq/ v2.0.0 fastQC Andrews et al.49 0.11.8 10x Genomics Cell Ranger (Software) Zheng et al.,50 10X Genomics 7.0.0 R (bulk and scRNA-seq blood analysis) https://www.cran.r-project.org v4.1.0 R (DNA methylation analysis) https://www.cran.r-project.org v4.2.1 R (scRNA-seq BAL analysis) https://www.cran.r-project.org v4.3.1 Seurat (scRNA-seq blood, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.3.0 Seurat (scRNA-seq BAL, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.4.0 Harmony (R package) Korsunsky et al.54 v0.1.0 ClusterProfiler (R package) Yu et al.55 v4.0.5 batchelor (R package) Haghverdi et al.56 v1.16.0 AUCell (R package) Aibar et al.57 v1.22.0 DESeq2 (R package) Love et al.58 v1.32.0 (Continued on next page) ll OPEN ACCESS Cell 187, 1–18.e1–e12, August 8, 2024 e3 Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article

    RNA Sequencing:

    Article Title: The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER BD Rhapsody cDNA Kit BectonDickinson Cat# 633773 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32851 High Sensitivity DNA Kit Agilent Cat# 5067-4626 NovaSeq 6000 S2 Reagent Kit (200 cycles) Illumina Cat# 20040326 NovaSeq 6000 S4 Reagent Kit (200 cycles) Illumina Cat# 20028313 Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat# 1000121 Chromium Next GEM Chip G Single Cell Kit 10x Genomics Cat# 1000120 Single Index Kit T Set A 10x Genomics Cat# 1000213 TruSeq Stranded Total RNA with Ribo-Zero Globin Illumina Cat# 20020613 Maxpar X8 Antibody Labeling Kit Fluidigm Cat# 201146B Quick-DNA Microprep Kit Zymo Research Cat# D3020 EZ DNA Methylation-Gold Kit Zymo Research Cat# D5006 Infinium MethylationEPIC BeadChip Kit Illumina Cat# WG-317-1002 Zombie Aqua Fixable Viability Kit BioLegend Cat# 423101 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit Becton, Dickinson Cat# 554714 SeraSpot Anti-SARS-CoV-2 IgG microarray Seramun Diagnostica SP-015-4 G-S12 RUO Deposited data scRNA-seq raw data This paper EGAS00001007461, EGAS50000000203 bulk RNA-seq raw data This paper EGAS00001007461 processed scRNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa processed bulk RNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa DNA methylation data This paper GSE270901 Code for DNA methylation data analysis This paper https://github.com/knollr/COVID_Dexa Software and algorithms Bcl2fastq2 Illumina v2.20 STAR Dobin et al.47 v2.7.3a Cutadapt Martin et al.48 v1.16 Dropseq-tools https://github.com/broadinstitute/Drop- seq/ v2.0.0 fastQC Andrews et al.49 0.11.8 10x Genomics Cell Ranger (Software) Zheng et al.,50 10X Genomics 7.0.0 R (bulk and scRNA-seq blood analysis) https://www.cran.r-project.org v4.1.0 R (DNA methylation analysis) https://www.cran.r-project.org v4.2.1 R (scRNA-seq BAL analysis) https://www.cran.r-project.org v4.3.1 Seurat (scRNA-seq blood, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.3.0 Seurat (scRNA-seq BAL, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.4.0 Harmony (R package) Korsunsky et al.54 v0.1.0 ClusterProfiler (R package) Yu et al.55 v4.0.5 batchelor (R package) Haghverdi et al.56 v1.16.0 AUCell (R package) Aibar et al.57 v1.22.0 DESeq2 (R package) Love et al.58 v1.32.0 (Continued on next page) ll OPEN ACCESS Cell 187, 1–18.e1–e12, August 8, 2024 e3 Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article

    DNA Methylation Assay:

    Article Title: The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER BD Rhapsody cDNA Kit BectonDickinson Cat# 633773 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32851 High Sensitivity DNA Kit Agilent Cat# 5067-4626 NovaSeq 6000 S2 Reagent Kit (200 cycles) Illumina Cat# 20040326 NovaSeq 6000 S4 Reagent Kit (200 cycles) Illumina Cat# 20028313 Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat# 1000121 Chromium Next GEM Chip G Single Cell Kit 10x Genomics Cat# 1000120 Single Index Kit T Set A 10x Genomics Cat# 1000213 TruSeq Stranded Total RNA with Ribo-Zero Globin Illumina Cat# 20020613 Maxpar X8 Antibody Labeling Kit Fluidigm Cat# 201146B Quick-DNA Microprep Kit Zymo Research Cat# D3020 EZ DNA Methylation-Gold Kit Zymo Research Cat# D5006 Infinium MethylationEPIC BeadChip Kit Illumina Cat# WG-317-1002 Zombie Aqua Fixable Viability Kit BioLegend Cat# 423101 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit Becton, Dickinson Cat# 554714 SeraSpot Anti-SARS-CoV-2 IgG microarray Seramun Diagnostica SP-015-4 G-S12 RUO Deposited data scRNA-seq raw data This paper EGAS00001007461, EGAS50000000203 bulk RNA-seq raw data This paper EGAS00001007461 processed scRNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa processed bulk RNA-seq count data and code This paper https://github.com/knollr/COVID_Dexa DNA methylation data This paper GSE270901 Code for DNA methylation data analysis This paper https://github.com/knollr/COVID_Dexa Software and algorithms Bcl2fastq2 Illumina v2.20 STAR Dobin et al.47 v2.7.3a Cutadapt Martin et al.48 v1.16 Dropseq-tools https://github.com/broadinstitute/Drop- seq/ v2.0.0 fastQC Andrews et al.49 0.11.8 10x Genomics Cell Ranger (Software) Zheng et al.,50 10X Genomics 7.0.0 R (bulk and scRNA-seq blood analysis) https://www.cran.r-project.org v4.1.0 R (DNA methylation analysis) https://www.cran.r-project.org v4.2.1 R (scRNA-seq BAL analysis) https://www.cran.r-project.org v4.3.1 Seurat (scRNA-seq blood, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.3.0 Seurat (scRNA-seq BAL, R package) Butler et al.,51 Hafemeister et al.,52 Stuart et al.53 v4.4.0 Harmony (R package) Korsunsky et al.54 v0.1.0 ClusterProfiler (R package) Yu et al.55 v4.0.5 batchelor (R package) Haghverdi et al.56 v1.16.0 AUCell (R package) Aibar et al.57 v1.22.0 DESeq2 (R package) Love et al.58 v1.32.0 (Continued on next page) ll OPEN ACCESS Cell 187, 1–18.e1–e12, August 8, 2024 e3 Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article



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